professional and amateur microscopy forum
This is a discussion on Fluorescence microscopy within the DIY Solutions forums, part of the Light Microscopes category; I have some UV and IR LEDs and would like to experiment using them but most scopes seem to use ...
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I have some UV and IR LEDs and would like to experiment using them but most scopes seem to use the light beam internally via a beam splitter/mirror scheme.
I would like to use them at a high angle (near parallel to objective +/- 30 deg.). Does anyone have a suggestion on (removeable) mounting these to or adjacent to the scope? |
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I was able to make a satisfactory super-stage lamp for use with IR and UV LEDs.
The most difficult part was the goose-neck lamp stand. I procured one from a thrift store which had a strong spring clamp on base. The materials used were: 3 LEDs of UV/IR type (Jameco) 1k ohm resistor 2pole, 6 position rotary switch and knob (a bit of overkill but all that was available) Power jack (tip & shank type) Machine tooled or dip socket to accept LED leads. Small scrap piece of prototype thru-the-hole printed circuit board (pcb) Spacers 2, 6-32 machine screws and 4 nuts Plastic box 3"h x 3"w x 1.7"d Plastic pill bottle 3.5" tall x 1.8" diam. Metal sample can 2.8" tall and fits snuggly into plastic bottle 4 wires carry power from the rotary switch and power input jack to the lamp socket. 3 wires are soldered to the rotary's perimeter contacts and a 4th to the ground of the power jack. The resistor is connected between the positive side of the jack and the center pole of the rotary switch. The socket is soldered to the pcb with one wire for the emitter side and separate wires for the anodes of the LEDs. The opposite wire ends are soldered to the LED socket after being fished through the goose neck, the side of the plastic bottle and metal can's bottom. The pcb is drilled for 2, 6-32 machine screws which are pushed through the pcb, spacers, and a nut before being pushed down into the can and through waiting holes and nuts there. The nut which formerly held the reflector on is used to keep the plastic bottle on the goose neck. The rotary switch and power jack are attached to a plastic box. The can's lid is given a 3/32" hole in its center to create a small beam. A wall wart or battery can supply the 9v needed. It satisfies my petrologic needs and required angle of beam. The aluminum can reflects the UV quite well but I did add a couple of small mirrors in the inside of the lid. Hope others find this useful. Geo1 PICTURES HERE: Last edited by geo1; 03-31-2008 at 02:06 AM. |
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Your note seems to have been truncated (or perhaps I'm truncated (mentally)) but if I got the drift of what you were saying was that the examination of any spectral data relies on the spectra of the illumination. -- and of course you are correct! LED illumination is not as narrow as the catalogs would infer by the stipulation of a nm frequency of illumination. In fact it can be all over the place. This is one of the reasons I am in the process of building a simple reflective/transmissive spectroscope, which at the moment is in hiatus while I concoct a means to position a scale in it (could buy it but this is more fun).
The fluorescent illuminator was constructed possibly out of jealousy of those with "in scope" means of fluorescent illumination. A profound failure by comparison but at least I can get some long wave capability with minimal effort. Let me know if I addressed this satisfactorily. |
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| Posted By | For | Type | Date | |
| Bio Heath Kit - DIYbio | Google Groups | This thread | Refback | 04-11-2008 03:08 PM | |