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Suggestions?

This is a discussion on Suggestions? within the Sample preparation forums, part of the Light Microscopes category; Hello, Does somebody has some ideas for me? I have just bought a microscope and I have considered some samples ...


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  #1 (permalink)  
Old 11-20-2009, 10:00 PM
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Hello,

Does somebody has some ideas for me?
I have just bought a microscope and I have considered some samples of sugar, NaCl and leafs, but I am wondering what to consider now.
So, does somebody has a suggestion for me?

Nadia.

(I come from the Netherlands and my english isn't very good, so i'm sorry if I make mistakes in my sentences.)
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Old 11-20-2009, 11:01 PM
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Get an onion and peel off a thin layer of live tissue, not the dried part. Mount it on a slide, and you should be able to distinguish cells and material flowing between/around them.

There is enough stuff living in pond/lake/stream/river water to keep you busy for a very long time. If you live near the ocean, then you have even more to examine.

Insects provide some pretty amazing viewing opportunities. If you're lucky, you might even find some ectoparasites living on them.
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Old 11-20-2009, 11:29 PM
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Thank you very much for your suggestions!
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Old 11-21-2009, 01:15 AM
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Oh, I forgot to mention pollen.
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Old 11-21-2009, 07:50 PM
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From the garden: Very thin cross sections of herbaceous plant stems and roots.
Try to slice the epithelium layer on the ventral(bottom) surface of a leaf and see if you can identify the stomata.

From the human body: Cells can be easily removed from the inside of ones cheek by gently swabbing with a cotton "qtip" (earbud). Also, blood, and hair (try and pull out the follicle too (ouch!)) make interesting microscopic targets.

From the lake: Fish scales have concentric ridges one can count under low magnification to determine the age of the fish, much like the rings in a tree. Fillamentous and free living algae

The possibilities are really endless.

Mintaka

Last edited by Mintaka; 11-21-2009 at 07:52 PM.
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Old 09-22-2010, 01:04 AM
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Quote:
Originally Posted by karlssoni View Post
Insects provide some pretty amazing viewing opportunities. If you're lucky, you might even find some ectoparasites living on them.
I am new to microscopy. I scooped up some water out of the bird bath in the garden looking for anything that might be living in there. A dead fly came in my 'sample' and when I looked at it, there were these little organisms looked like they were eating it? I took a pic but it doesnt show as clear as it was with the eye.

I have yet to try some pond water, but I look forward to it!

John.

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Old 09-22-2010, 01:12 AM
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Just a guess, since I can't zoom in for a closer look (I tried -- silly me!), they look like rotifers using the fly as substrate, not food.

<http://en.wikipedia.org/wiki/Rotifer>
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Old 09-22-2010, 02:02 AM
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Thanks John, I will have a read about them.

Regards, John.
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Old 09-22-2010, 09:28 PM
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Hi John,

I agree with John K. They look like rotifers to me also. They will be feeding on bacteria which are feeding on the fly. If you look at them with a higher power objective you will see a crown of cilia beating o the top of them giving the appearance of a spinning wheel.

Peter.
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Old 04-13-2011, 12:41 AM
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Pond water--stuff some dry weedy grass in your specimen container (Empty rinsed out medication containers work well) and let it grow. Check daily. Add water as needed. WOW!
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