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Preserving Protists - Lugol's solution

This is a discussion on Preserving Protists - Lugol's solution within the Sample preparation forums, part of the Light Microscopes category; Rene Van Wezel comments at microscope yahoo group about a solution for conserving protists when travelling: In the drugstore. Also ...


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Old 01-03-2008, 02:11 PM
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Default Preserving Protists - Lugol's solution

Rene Van Wezel comments at microscope yahoo group about a solution for conserving protists when travelling:

Quote:
In the drugstore. Also let them make up an solution of 5 gr iodine,
10gr potassium iodide and 5 g of sodium acetate in 100 ml of water
(Lugol's solution). Use this in samples until tea colour (roughly 2-
4ml per liter sample). This stuff is relatively harmless and
preserves tiny flagellates better. Lugol samples will keep well for
at about month at room temperature in the dark. Formaldehyde samples
keep a lot longer, but both type of samples should be stored in the
fridge for long term storage (1 yr and longer).

Rene.
Link to wikipedia: Lugol's iodine - Wikipedia, the free encyclopedia
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Old 01-07-2008, 04:08 AM
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Yes you can'quick fix protists' while on holiday.Please consider'tinygrab&gomicroscope kit'! If your going to carry fixative solution in vials(would seem very suspicious to me these caustic/foul smelling vialed solutions..these are poisons/not part of 'personal hygiene shave /travel bag'..hard to explain at airport or any official 'search'.)'if your going to physically 'collect specimen samples'...fun to peek at them in their natural colors/natural behavior patterns/natural attachments. Dead stuff,sediment viewed back home..fun to see months latter, if the live protists you'field observed' thrive at home.
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Old 04-28-2008, 01:33 AM
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Default kit

What is a tinygrab&gomicroscope kit'
Thanks
Steve
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Old 04-28-2008, 10:09 AM
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Hi centratus, I think that he means something like that (please, correct me if I'm wrong)

mini microscope, Educational, Healthcare, Lab Life Science items on eBay.com

That kind of small microscopes that you can take with you. They are small and bad quality, but you can take a look as you go.

Greetings
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Old 04-28-2008, 05:34 PM
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Hello Miguel and centratus, happyspring time all! A relaxing 'added demension' to any vacation to a far from home location, is to note the difference in night skies (if ever I travel south again to Mexico, or Puerto Rico...you KNOW I will at night hope to look up and see :'Crux', the southern cross!). Diurnal enjoyment allows 'as you relax 'on holiday', ...alows note of the different vegetation/different birds,etc.,. A WELL PACKED KIT containing a small 'field microscope', a 'well slide' glass slide, a few regular slides, cover slips, lens paper and 70% 'rubbing alcohol, a pair of tweezers/ an eye dropper with rubber bulb...you are set for at least one relaxing evening, while your travel companion perhaps watches a television soap opera, the two of you sipping regional wine/ the 'spirits' of the region your visiting...you are set to OBSERVE LOCAL AQUATIC PROTISTS at the hotel room your staying in! From time to time call out with enthusiasm: "eegads...honey, come look at this suctorian! It may be a Tokophyra species?!! It's water sample from the public gardens we lunched in today, after the royal art museum!". Of course, ask her to when she gets up , to please bring some more wine out of the ice-bucket, to refill your glass. This in essence, THE SIMPLE JOY OF A 'GRAB and GO', portable/ field microscope kit. For added fun, in this forum, we could each explore if :1) putting your cell-phone camera lens up to the microscopes eyepiece, allows a :'vacation-micrscope photo'? My own 'learning-curve is simply with using the computer (which is why I have'nt indented in proper paragraph format, this posting,gang!) I applied a low cost ($99 dollar/US) handheld digital microscope, over the eyepiece of a 'vintage 1940's' Spencer/Buffalo monocular scope...ThE SYSTEM WORKS, but the digital pictures are in:'BitMap' format, and to share on internet in forums>>I need to "convert to JePeg format"...always the computer literacy which I seem to 'put off learning'!?? happy spring time, charlie guevara, NJ,US
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