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preparing slides

This is a discussion on preparing slides within the Sample preparation forums, part of the Light Microscopes category; If I'm going to look at some pond water and I want to see all the creepies swimming about, ...


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Old 11-30-2008, 07:36 PM
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Join Date: Nov 2008
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Default preparing slides

If I'm going to look at some pond water and I want to see all the creepies swimming about, do I or don't I put on a cover slip? I think without the cover slip, I won't be able to focus (or if I get too close, the lens will get pond water on it) but with the cover slip, all the creepies will get stuck/squashed.

How is this generally done?

Thanks,
Adam O.
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  #2 (permalink)  
Old 11-30-2008, 09:26 PM
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For good results observing pond water place a drop of water taken from your sample source where there is some debris. If you let the sample container sit quietly much of the debris will settle to the bottom of the container and you can get a dropper full and put it on the slide. Place the cover slip on the drop. If there is water coming out from under the coverslip around the edges you can wick it away with a piece of tissue. The water will suspend the organisms and the coverslip will not crush them. The coverslip will keep the slide from drying for a while and as you said, protect the objective from getting wet.

g2b2
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