Microscope Forums .com

Hello Guys,

I was wondering if anybody has performed an accuracy study on a microscope to make accurate length measurements. For example some formal way of saying to the customer that the length we measured is X with the actual value being +-1 % of the observed value X. I performed Type I Gage Study to assess the microscope's ability to make accurate length measurements but I have never performed any formal study to make a statement like I mentioned above. Any help is appreciated.

For verification purposes, you should ultimately compare the scale on your microscope eyepiece or monitor to something of a known size. For example, you could bring the graduations on a transparent plastic ruler into focus and measure the distance. Or compare against a nylon or steel screen of known mesh size.

There is no scale on the microscope eyepiece or monitor. The microscope is attached to a computer where the image is displayed.

How about measuring (with a ruler) the display size of an object of known size on your monitor to calculate the factor for that specific magnification?

The most reliable way to calibrate the microscope is to use a certified stage micrometer and a good quality ocular micrometer. Since each eyepiece and objective can give a slightly different result each combination must be measured separately. If you are giving reliability information to a customer this is the only way to do it. If you are looking for general measurements you can use the ruler and do the math.

Regards,
g2b2

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A pixel is worth a thousand words but it takes a thousand words to explain a pixel. g2b2

g2b2 is absolutely correct. It's very simple to set up, and once you've done it, it's done.

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John Karlsson
Hope Valley, RI

Don't count your chickens until they have burned their bridges behind them.