professional and amateur microscopy forum
This is a discussion on MerryChristmas,HappyNewYear group! within the Digital Camera Macro Pictures forums, part of the Image Galleries category; Best wisheshes for healthy and productive 2009. I hope we can share microscopy in this forum. charlie guevara NJ,US...
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I finally organized my microscope bench to hastily image a desmid. This is my 'first light' with that :relay-lens camera/microscope adaptor.
I set the Canon rebel-xt dslr to:'M' setting. Viewed and 'attempted focus' through the camera small view-finder. Gently pressed the shutter-button for an image. It worked! This desmid 'holds up well' to being magnified by your computer screens to: 400% . As all can see, there is a piece of spiral LINT...somewhere in the optical train of len surfaces. I have to 'roll up my sleves' and carefully clean all the lens surfaces. First thing I'll do is rotate the camera on the trinoc microscope tube conection. Dust...I have to order quite a few large-size microscope 'dust covers'. For years I've been using clean plastic bags...but here my Christmas card 'snowflake desmid' throws a long spiral piece of LINT in my images! You in this group can now keep me honest...if you see that piece of lint at:12-1 o-clock position in image fields...you know I did not yet find it and clear it up. charlie guevara NJ,US (ps) the flash image of my microscope shows every little 'pit and scratch' on this circa 1960's workhorse microscope...it sure looks like dust, but mostly that is not dust in the image! |
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I did not setout to do this....but!!!...by the way I gently cleared the desmid of debris..any by where it is relative to the clumped debris on that droplet of water...YOU CAN SEE that desmid...soflty green colored 'speck' centered in the wetmount slide imaged on the microscope stage!
Comparing 'back and forth' this droplet image, and the desmid image...you can see the orientations and various specific debris clumps surrounding the desmid...and of course you can with confidence 'SEE ' the softly green colored speck, which is that desmid. Somebody tell me to 'snap out of this naked eye testing'...tell me to:'get a life'! charlie guevara NJ,US |